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. 2006 Jul 18;103(30):11118–11123. doi: 10.1073/pnas.0604873103

Fig. 3.

Fig. 3.

The activity of Topo IIIα and Blm is specifically stimulated by RPA. The amount of resolution of the DHJS increases with increasing concentrations of hRPA in the reaction (compare lane 2 with lanes 3–5), whereas hRPA alone has no activity on the substrate (lane 6). This stimulation was specific to RPA; including other ssDNA-binding proteins, E. coli SSB (lanes 7–9) or T4 gp32 (lanes 11–13), showed no stimulation of the resolution of DHJS by Topo IIIα and Blm. The supplemented proteins are at 44, 88, and 175 nM concentrations, respectively.