Fig. 3.

Methylation status of the H19 differentially methylated domain in control and sex-reversed male and female germ cells. (a) Schematic representation of sequences upstream of the H19 promoter. The double-headed arrow indicate the region in which methylation was analyzed. (b and c) Each line corresponds to a single strand of DNA, and each circle represents a CpG dinucleotide on that strand. The number of strands observed with a given methylation profile (if greater then one) is indicated to the left of each line. Nineteen CpGs were analyzed by bisulphite mutagenesis and sequencing. A filled circle represents a methylated and an open circle an unmethylated cytosine. Analyses were performed on male and female germ cells at 15.5 dpc (b) and 16.5 dpc (c). XY + Sry, male control group; XX, female control group; XY-Sry, experimental XY female group; XX + Sry, XX male group, sterile.