Growth and the β-galactosidase activity of the biofilm-defective S. gordonii adcR::Tn917-lac mutant at 0, 3, 3.5, 4. 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8.5, and 24 h are shown. Cultures were grown at 37°C under anaerobic conditions in THBYE. The β-galactosidase activity of sonic extracts from each time point was quantified with MUG (28). All assays were performed in triplicate, and mean values and standard deviations are shown. MU, 4-methyl-umbelliferone.