Fig. 4.
Validation by PCR of several probes containing possible SNPs identified from the comparison of C57BL/6 to BALB/c. Fragments were amplified such that they were larger than the genomic BglII representational fragment and digested with BglII to detect the presence of SNPs in one or both BglII cleavage sites. C57BL/6, fragment amplified from C57BL/6; C57BL/6-D, fragment amplified from C57BL/6 digested with BglII; BALB/c, fragment amplified from BALB/c; and BALB/c-D, fragment amplified from BALB/c digested with BglII. The arrows point to the fragment being queried. The ratio is plotted on the y axis in log scale, and the x axis is a genome order. The markers on the gel picture (M) are a 100-bp ladder.