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. 2006 Jul 14;103(30):11246–11251. doi: 10.1073/pnas.0604657103

Fig. 3.

Fig. 3.

Analysis of Dazl-shRNA phenotype. (A–C, E–G, and I) Hematoxylin/eosin histological staining of testis (A, B, E, and F) and epididymis (C and G) from an adult (9-week-old) Dazl-shRNA rat (E–G) and a wild-type sibling (A–C). (D and H) CREM immunofluorescence (red) and overlaid DAPI staining (blue) in testis of a young (6-week-old) Dazl-shRNA rat (H) or a wild-type sibling (D). [Scale bars: 50 μm in A (for A, C–E, and G–I) and 10 μm in B (for B and F).] (I) Hematoxylin/eosin histological staining of testis from a 6-month-old Dazl-shRNA rat. (J) Testis and epididymis from 6-month-old wild-type (Upper) or Dazl-shRNA (Lower) rat. (K) Graphical representation of mean testis weight (in grams) at various ages. The following numbers of testes were examined: at 6 weeks old, 4 wild-type and 10 transgenic; at 7 weeks old, 2 wild-type and 6 transgenic; at 9 weeks old, 2 wild-type and 4 transgenic; at 11 weeks old, 4 wild-type and 10 transgenic; at 26 weeks old, 2 wild-type and 4 transgenic.