Table 1.

Fecundity and germ-line transmission of transgenic founders

Founder		GFP expression*		Litter size		Transmission†
No.	Sex	Overall	Relative	Mean	No.	Tg/total F1	%
8-8	M	−	1.1	12	3	0/36	0
16-13	M	−	1.1	9	1	5/9	55
15-12	M	−	1.2	11	2	0/21	0
16-4	F	−	1.3	16	2	0/32	0
16-14	M	+	1.7	11	5	0/48	0
17-11	M	+	1.8	10	7	0/69	0
17-9	F	+	1.9	11	5	28/54	52
16-15	F	+	2.1	8	5	0/27	0
1-9	M	+	2.8	13	4	0/48	0
15-10	F	+	2.9	11	5	0/56	0

M, male; F, female; Tg, transgenic.

*Overall presence of detectable GFP expression was assessed by comparing fluorescence in transgenic rats with wild-type rats in 2-mm punches of ear skin using a stereoscope. Relative amounts of GFP expression were estimated by using imagej software to obtain a transgenic-to-wild-type ratio of fluorescence in an ear punch for each founder.

^†F₁ of total progeny genotyped by using PCR.