TABLE 1.
Bacterial strains, phage, and plasmids
| Strain, phage, or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| KH5402-1 | thyA thr trpE9829(Am) ilv tyrA(Am) metE deo supF6(Ts) | T. Katayama 19 |
| KA413 | KH5402-1 dnaA46 ilv+ | 11 |
| KA413ΔH | KA413 aphA::Km | This study |
| M2-24 | Temperature-resistant revertant of KA413ΔH | This study |
| M2-5 | Temperature-resistant revertant of KA413ΔH | This study |
| M2-11 | Temperature-resistant revertant of KA413ΔH | This study |
| JM109 | 28 | |
| C600 | 1 | |
| C600 dam | C600 dam-13 | This study |
| C600 yccV | C600 yccV::mini-Tn10 | This study |
| C600 dam yccV | C600 dam-13 yccV::mini-Tn10 | This study |
| WM1032 | dnaA508 ilvB or ilvG lac supD thi trp | W. Messer |
| WM1032 yccV | WM1032 yccV::mini-Tn10 | This study |
| WM2007 | TC3145 lac his RB1 proB902 rpsL supF81(Ts) thi trp(Am) tsX | W. Messer (RB220 derivativec) |
| WM2007 yccV | WM2007 yccV::mini-Tn10 | This study |
| Phage | ||
| λ1098 | Pam80 cI857(Ts) nin5 mini-Tn10 | 27 |
| Plasmids | ||
| pSK+ | Bluescript, pBR322 derivative vector | Stratagene |
| pUC18 | pBR322 derivative vector | 28 |
| pSK24 | 6.7-kbp Pstl chromosomal fragment carrying yccV::mini-Tn10 cloned on pSK+a | This study |
| pSK11 | Deletion part of a Pstl chromosomal fragment carrying mutS::mini-Tn10 cloned on pSK+ | This study |
| pUC5 | Deletion part of a Pstl chromosomal fragment carrying rpoN::mini-Tn10 cloned on pUC18 | This study |
| pDK5 | pBR322 derivative expression vector | 13 |
| pES6ΔHE | Derived from pDK5 by inserting between EcoRI and HindIII two complementary oligonucleotides, generating an XbaI site flanked by a hexahistidine coding sequence | This study |
| pES6ΔHEE1 | RBS from PT7 and YccV ORF cloned between the EcoRI and XbaI sites of pES6ΔHEb | This study |
a
The fragment was from PstI position 8776 of GenBank sequence ae000198.gb_ba2 to PstI position 1008 of GenBank sequence ae000199.gb_ba2.
b
RBS, ribosome binding site; ORF, open reading frame.
c
See reference 5.