Fig. 2.

Effects of TER 180 Rz expression on B16 cell morphology and expression of tyrosimase and Melan-A. (A) Flasks containing confluent cell cultures of ribozyme clone 5 (28.5), pooled ribozyme (P28), and pooled vector (P35) B16 clones with medium. (B–D) In vitro morphology (Left) of ribozyme clone 5 (B), pooled ribozyme (C), and pooled vector (D) B16 clones, along with tyrosinase (Center) and Melan-A (Right) immunostaining for each subclone. (E) Western blot of tyrosinase and actin in pooled vector (lanes 1, 4, 7, and 10), pooled ribozyme (lanes 2, 5, 8, and 11), and clone 5 (lanes 3, 6, 9, and 12) cells on day 0 (lanes 1–3), day 3 (lanes 4–6), day 7 (lanes 7–9), and day 10 (lanes 10–12).