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. 2006 Jul 14;103(30):11340–11345. doi: 10.1073/pnas.0602127103

Fig. 4.

Fig. 4.

Filter-binding assays of purified FljA binding to 5′ UTR duplication mutants and single-base mutants. (A) Assay of purified His6-FljA binding to in vitro-transcribed RNA from the following fliC 5′ UTR mutants: −62 to +15 bp from AUG of fliC for WT and the fliC duplication mutants DUP-(−15 to −19) and DUP-(−13 to +15). Because DUP-(−13 to +15) is a longer transcript, a second WT transcript, WT −62 to +26, was used as a control. (B) Assays of purified His6-FljA binding to in vitro-transcribed RNA from WT, −24G → C, −4G → U, and Δ(−13U).