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Diagram illustrating the principle of M-PFC. Interacting proteins A and B are fused to complementary mDHFR fragments F[1,2] and F[3]. Cotransformation of AF[1,2] and BF[3] fusions in Msm results in the functional reconstitution of mDHFR activity and subsequent growth on TRIM plates, whereas proteins that do not interact (A and C) will not reconstitute F[1,2] and F[3] and will not allow transformants to survive on TRIM plates.
