Table 1.
Permeation and gating properties of V166E rClC-K1 channels coexpressed with WT and mutant barttin or without barttin, respectively
| Pore-forming and accessory subunit | Unitary conductance, pS | V0.5, mV | Apparent gating charge, eo | Popenmax | Popenmin |
|---|---|---|---|---|---|
| V166E rClC-K1 | 6.5 ± 0.3, n = 11 | −110.2 ± 2.8, n = 8 | −1.0 ± 0.0, n = 8 | 1.0 | 0.14 |
| + Y57X barttin | 9.1 ± 0.3, n = 6 | 31.2 ± 1.4, n = 16 | 1.1 ± 0.1, n = 16 | 0.32 | 0.19 |
| + T61X barttin | 13.1 ± 0.5, n = 7 | 38.0 ± 2.3, n = 12 | 1.3 ± 0.1, n = 12 | 0.55 | 0.27 |
| + I72X barttin | 18.1 ± 0.4, n = 12 | 17.7 ± 1.9, n = 19 | 1.1 ± 0.0, n = 19 | 0.88 | 0.21 |
| + L85X barttin | 16.6 ± 0.6, n = 9 | 15.3 ± 2.3, n = 14 | 1.1 ± 0.0, n = 14 | 0.71 | 0.19 |
| + S115X barttin | 18.7 ± 0.6, n = 7 | 11.4 ± 1.3, n = 17 | 1.2 ± 0.0, n = 17 | 0.84 | 0.33 |
| + L243X barttin | 18.5 ± 0.7, n = 10 | 14.7 ± 2.8, n = 6 | 1.0 ± 0.1, n = 6 | 0.87 | 0.30 |
| + WT barttin | 19.4 ± 0.9, n = 6 | 11.6 ± 3.5, n = 15 | 0.9 ± 0.0, n = 15 | 1.00 | 0.20 |