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Western blot analysis (using polyclonal antibody to the R. erythropolis porin) of the cell wall porins of R. erythropolis and R. equi. Positions of size markers are shown on the right. The channel-forming protein PorAReq was extracted as described in Materials and Methods, and the cell wall channel of R. erythropolis was determined as described previously (22). Lane 1, cation-selective channel-forming protein of R. equi (PorAReq); lane 2, channel-forming protein of R. erythropolis.
