TABLE 1.
Bacterial strains, plasmids, and phages
| Strain, plasmid, or phage | Relevant propertiesa | Source or Reference |
|---|---|---|
| E. coli | ||
| LJ110 | Alias W3110, wild-type K-12, prototroph | J. Lengeler |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 fruA25 | 7 |
| CK1953 | MC4100 secB::Tn5 | C. Kumamoto |
| DADE | MC4100 ΔtatA-tatD ΔtatE | 42 |
| MM129 | F−malT1c tsr-1 rpsL136 ΔmalE444 zjb729::Tn10 | 5 |
| GSJ100 | MC4100 × P1.MM129>Tcr ΔmalE444 zjb729::Tn10 | This study |
| GSJ101 | DADE × P1.MM129 > Tcr ΔmalE444 zjb729::Tn10 | This study |
| Plasmids | ||
| pMMB67EH | RSF1010-derivative, tacP, rrnB; Apr | 15 |
| pBBR1MCS-2 | mob, rep, lacZα+; Kmr | 25 |
| pBBR2MCS-2 | mob, rep, lacZα+; Apr | This study |
| pHSG575 | pSC101 replicon, lacZα+; Cmr | 40 |
| pBluescriptKS | lacZα+ cloning vector; Apr | Stratagene |
| pTatABC | pHSG575 derivative; carrying the tatABC operon of E. coli | This study |
| pMalE | pBBR2MCS-2 derivative; carrying the pre-malE gene | This study |
| pTorA-MalE | pBBR1MCS-2 derivative; carrying the torA-malE fusion gene | This study |
| pATorA-MalE | pBBR2MCS-2 derivative; carrying the torA-malE fusion gene | This study |
| pTorA*-MalE | pBBR1MCS-2 derivative; carrying the torA*-malE mutant fusion gene | This study |
| pATorA*-MalE | pBBR2MCS-2 derivative; carrying the torA*-malE mutant fusion gene | This study |
| pMTorA-MalE | pMMB67EH derivative; carrying the torA-malE fusion gene | This study |
| Phages | ||
| λTnphoA-1 | b221 cI857 Pam3::TnphoA Kmr | 17 |
| Plkc | Transducing phage | Laboratory stock |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance.