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. 2006 Jul 11;103(29):10911–10916. doi: 10.1073/pnas.0604496103

Fig. 3.

Fig. 3.

Ouabain specifically up-regulates the expression of connexin 32. (A) Western blot of connexins 43, 32, and 26 in R/W monolayers with or without 1.0 μM ouabain for 24 h. (B) By using the band of actin to normalize the density of connexin bands, ouabain was found to enhance the expression of connexin 32 without affecting the expression of Cx43 and Cx26. Immunofluorescence images of mixed monolayers show that each connexin distributes according to a particular pattern (C, E, and G under control condition), which is disarranged by 1.0 μM ouabain (D, F, and H). (I) Expression of connexin 32 in monolayers of R cells.