Skip to main content
. 2006 Jul 11;103(29):10911–10916. doi: 10.1073/pnas.0604496103

Fig. 4.

Fig. 4.

Effect of ouabain on the P→A mechanism. (A, D, and G) Immunofluorescence images of the β-subunit of the Na+,K+-ATPase, occludin, and β-catenin in W cells under control conditions. (B, E, and H) The same markers as affected by 1.0 μM ouabain for 12 h (B and E) or 8 h (H). Nuclei in H were stained in red with propidium iodine. (C, F, and I) The same markers in monolayers of mixed W and R cells (red) treated with ouabain for 24 h. Note that in B, E, and H we used a shorter incubation time to observe the distribution of markers in W cells because this cell type would not have withstood this concentration of ouabain for 24 h. (J) Western blots of occludin and β-catenin in pure monolayers of W or R cells or in cocultures of W/R cells under control and 24 h of ouabain treatment. (K) TER under the same experimental conditions.