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Two ClpXP substrates, CtrA and the polar McpA chemoreceptor, depend on CpdR for their proteolysis. (A) Immunoblots of synchronized populations of wild-type, ΔcpdR::Ω, ΔcpdR::Ω with the complementing plasmid pcpdR, and cpdRD51A strains by using antibodies to CtrA and McpA. A cell cycle schematic is shown. (B and C) Pulse–chase experiments showing the half-lives of CtrA (B) and McpA (C) in wild-type, ΔcpdR::Ω, and cpdRD51A strains.
