Skip to main content
. 2006 Jul 10;103(29):11033–11038. doi: 10.1073/pnas.0600829103

Fig. 2.

Fig. 2.

Staining of B. subtilis PY79 with ramoplanin analogs. (A) Examples of sidewall staining observed with 2a:2b. (B–H Left) Probe-treated cells. (B–H Center) TMA-DPH-treated cells (mb, membrane stain). (B–H Right) Overlays of probe-treated (green) and TMA-DPH-treated (red) cells. Arrowheads and arrows point to old division sites (poles) and new division sites (septa), respectively. (A) Fluorescent images of 1:1 mixture of 2a:2b at 0.5 μg/ml each, along with a schematic representation of the helix. (B) 2b at 0.1× MIC (2 μg/ml). (C) 2b at 1.0× MIC (20 μg/ml). (D) A 1:1 mixture of 2a:2c at 0.5 μg/ml each (higher concentrations look similar). (E) A 1:1 mixture of 2a:2b at 0.5 μg/ml each. (F) A 1:1 mixture of 2a:2b at 1.0 μg/ml each. (G) A 1:1 mixture of 2a:2b at 2.5 μg/ml each. (H) 2d at 0.1× MIC (1.0 μg/ml). (Scale bars, 2 μm.) See Fig. 5, which is published as supporting information on the PNAS web site, for larger fields.