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. 2006 Jul 10;103(29):11051–11056. doi: 10.1073/pnas.0604548103

Fig. 2.

Fig. 2.

The proline-rich region and the FLAG tag can alter polyQ toxicity in trans. (A) Cells coexpressing the indicated htt exon I proteins or a vector control were spotted on plates that either induce (+Induction) or repress (−Induction) protein expression in three 5-fold dilutions. Toxicity is reflected by reduced growth of cells under inducing conditions. (B) A dot blot was performed with protein lysates from cells expressing the indicated combinations of different htt exon I proteins. The blot was probed with an anti-FLAG antibody. (C) Cells containing FLAGhtt103QΔProCFP and either a vector control or the indicated 25Q htt exon I proteins were spotted on plates that either induce (+Induction) or repress (−Induction) protein expression in three 5-fold dilutions. (D) Cells containing FLAGhtt25QΔProCFP and either a vector control or the indicated 103Q htt exon I proteins were spotted on plates that either induce (+Induction) or repress (−Induction) protein expression in three 5-fold dilutions.