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. 2006 Jul 10;103(29):11075–11080. doi: 10.1073/pnas.0604246103

Fig. 2.

Fig. 2.

Functional characterizations of Am4′CGT. (A) A phylogenic tree for the flavonoid-related and 18 A. majus glucosyltransferases constructed by a clustal-w program (32), macvector 7.2.2 software (Accelrys, San Diego). The number on the branches indicates sequence difference (0.05 corresponds to a 5% change). UGT88A1 (AAN28841) is an Arabidopsis, function unknown, glucosyltransferase; Am3GT is an Antirrhinum anthocyanidin 3-O-GT; Db5GT (Y18871) is a Dorotheanthus betanidin 5-O-GT; and Gt3′GT (AB076697) is a Gentiana anthocyanin 3′-O-GT. (B) Expression patterns of Antirrhinum flavonoid-related structural genes during flower development. L, leaves; numbers above the lanes, a previously defined developmental flower stage (10). (C) Enzymatic activity of recombinant Am4′CGT protein against THC and PHC. Chromatograms show the absorption at 360 nm in each reaction mixture. HPLC condition is described in Materials and Methods. Standards were eluted at retention times (R.T.) 24.3 (PHC 4′-O-glucoside), 27.7 (THC 2′-O-glucoside), 30.0 (THC 4′-O-glucoside), 32.4 (PHC), and 38.2 min (THC). Arrows indicate the THC 4′-O-glucoside and PHC 4′-O-glucoside synthesized, respectively, by Am4′CGT from THC and PHC.