Table 1.
Real-time PCR confirmation of cytokinin-regulated microarray results
| Gene name | At number | Wild type + vs. − BA |
crf1,2,5 + vs. − BA |
crf2,3,6 + vs. − BA |
|||
|---|---|---|---|---|---|---|---|
| Microarray | RT-PCR | Microarray | RT-PCR | Microarray | RT-PCR | ||
| Zinc Finger B-box | At1g68520 | 3.1 | 2.8 ± 0.3 | 1.0 | 1.3 ± 0.1 | 1.1 | 1.1 ± 0.1 |
| NAM AtNAC6 | At4g27410 | 2.7 | 2.7 ± 0.2 | 1.3 | 0.7 ± 0.1 | 1.4 | 1.0 ± 0.1 |
| Speckled POZ | At3g48360 | 3.5 | 2.7 ± 0.3 | 1.1 | 0.8 ± 0.1 | 0.9 | 1.0 ± 0.5 |
| Senescene Assoc. P. | At1g53885 | 3.9 | 2.7 ± 0.3 | 1.3 | 1.7 ± 0.1 | 2.0 | 1.5 ± 0.3 |
| ARR5 | At3g48100 | 7.3 | 14.3 ± 0.4 | 3.2 | 26.6 ± 1.1 | 5.0 | 25.4 ± 0.9 |
| ARR7 | At1g19050 | 3.6 | 9.5 ± 0.3 | 5.7 | 15.1 ± 0.6 | 5.5 | 12.1 ± 0.4 |
Four genes regulated by cytokinin in wild type, but not in CRF mutants, were examined by using real-time PCR, as well as two type-A ARR genes and show similar trends to the microarrays in Fig. 4A.