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. 2006 Jul 24;103(31):11509–11514. doi: 10.1073/pnas.0604988103

Fig. 2.

Fig. 2.

Preparation of Nanodisc-embedded chemoreceptor Tar-6H. (a) Coomassie-stained SDS-polyacrylamide gel of samples (equivalent proportions of the total material at each stage) from stages of Nanodisc preparation with chemoreceptor (Tar; 60 kDa) and MSP (30 kDa) indicated. (b) Size-exclusion chromatography of Nanodiscs containing only lipid (Discs) or lipid plus chemoreceptor (Discs + Tar). (Upper) Continuous recordings of absorbance. (Lower) Coomassie-stained SDS-polyacrylamide gels of samples from fractions collected. (c) Tar polypeptides per disk as a function of preparation ratio of scaffold protein to receptor determined by quantification of bands like those in a.