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Phosphorylation of GST-tagged Mcm2 N-terminal peptides. (A) Amino acid sequence of the Mcm2 N terminus and peptide regions fused to GST. (B) GST-tagged Mcm2 N-terminal peptides (A–F, 1 μg, ≈30 pmol) or full-length Mcm2 protein (Full, 0.3 μg, 3 pmol) were phosphorylated with Cdc7 kinase (+, 5 ng; ++, 15 ng) at 30°C for 30 min. The mixtures were subjected to SDS/PAGE followed by staining with Coomassie blue (Upper) and autoradiography (Lower).
