Fig. 4.

Liprin-α is not essential for LAR localization in S2R+ cells. (A–F) Liprin-α and LAR colocalize with the focal adhesion marker Talin in S2R+ cells. S2R+ cells were transfected with HA-Liprin-α (A–C) or HA-LAR (D–F) and actin5c-GFP (blue in A and D) as a transfection marker. Cells were fixed and stained with anti-HA (B and E; red in A and D) and anti-Talin (C and F; green in A and D). (G–I) Liprin-α RNA interference did not alter LAR colocalization with Talin. S2R+ cells were transfected with HA-LAR and a double-stranded RNA hairpin construct directed against Liprin-α, and stained with anti-HA (H, red in G) and anti-Talin (I, green in G).