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Characterization of the human T cell clone. (A) Proliferation of the human P-Ins73-90-specific T cell clone, 52c1 to 21 overlapping preproinsulin (PP-Ins) peptides (16 mers; on x axis). (B) Cytokine secretion of the clone 52c1. In A and B, autologous BMNC were used as APC. (C) T cell clone was tested on different concentrations of P-Ins, insulin (Ins), C-peptide (C-pep), and P-Ins73-90 peptide by using allogeneic homozygous DR4 dendritic cells as APC.
