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. 2006 Jul 20;103(31):11707–11712. doi: 10.1073/pnas.0603806103

Fig. 1.

Fig. 1.

ALDH inhibition impedes the differentiation of human HSCs. FACS-sorted CB CD34+CD38−lin− cells were analyzed for ALDH activity levels and compared with the ALDH activity level in the CD34+CD38− progeny of 7-day cultures with thrombopoietin, SCF, and flt3-ligand (TSF) alone versus TSF + DEAB (a). (b) The surface expression of CD34 and CD38 on day 0 CB CD34+CD38−lin− cells (Top) and their progeny after culture with TSF alone (Middle) versus TSF + DEAB (Bottom) is shown. Culture with TSF alone caused a marked increase in CFC content compared with day 0 CD34+CD38−lin− cells, whereas the progeny of DEAB + TSF cultures contained little CFC activity, indicating an inhibition of HSC differentiation during culture (c). ∗ indicates a statistically significant difference between the DEAB + TSF treated group versus TSF alone.