Fig. 4.

Treatment with DEAB sustains HOXB4 expression in HSCs. RNA was isolated from multiple replicates of FACS-sorted CB CD34⁺CD38⁻lin⁻ cells at day 0 and their progeny after culture with TSF alone or DEAB + TSF. The RNA was reverse-transcribed, and the expression of HOXB4 and Notch 1 was analyzed by quantitative real-time PCR. (Left) The expression of HOXB4 in CD34⁺CD38⁻ cells was significantly reduced after short-term culture with TSF, whereas treatment with DEAB prevented the down-regulation of HOXB4 expression over time. (Right) The expression of Notch was also significantly reduced after TSF culture and was not altered by treatment with DEAB.