Fig. 4.

Phenotypic analysis of hESC-derived thymocytes. (a) To distinguish endogenous HLA-A2⁻ and hESC-derived HLA-A2⁺ cells, thymocytes from control mice (Left) and SCID-hu mice into which hESC-derived CD34⁺ cells had been transferred (Right) were stained and gated based on the expression of CD45 and EGFP. MFI, mean fluorescence intensity. Cells within each gate were subsequently analyzed for the expression of HLA-A2. (b) CD45⁺, EGFP⁺ cells from the Thy/Liv implants from the RAG-hu mice transferred with hESC-derived CD34⁺ cells (Upper) and CD45⁺ cells from the implants of the control animals (Lower) were gated and analyzed for the presence of the indicated T cell surface markers. Region markers are based on isotype controls.