Fig. 1.

Expression of Mbnl1 isoforms in skeletal muscle. (A) Distribution of Mbnl1 isoforms in neonatal (P2) and P28 muscle. Ten hindlimb muscle isoforms were identified by RT-PCR followed by cDNA sequencing. Isoforms (color coded) are as follows (in kDa): 43, lime green; 42, black; 41, turquoise; 40, orange; 38, purple; 37, light blue; 36, red; a 36 spliced variant (36*, dark green); 35, yellow; and 32, blue. (B) The major Mbnl1 protein in adult skeletal muscle is 40 kDa. Immunoblot analysis was performed on three skeletal muscles (tibialis anterior, gastrocnemius, and quadriceps), cerebellum, heart, and lung by using either anti-Mbnl1 or anti-Gapdh (loading control).