Fig. 4.

Isolated mce mutants verify the interactions predicted by using TraSH. Each mutant was mixed with a similar number wild type H37Rv, inoculated intravenously into C57BL/6 (A and C) or BALB/c (B) mice, and organs were harvested at the indicated time points. (A and B) The ratio of mutant/wild-type bacteria in the spleens of infected mice plotted over time. (C) Colony-forming units of ΔmceG and wild-type bacteria in the lungs of infected mice plotted individually (same data as A). Log scales are used on each y axis. All mutants grew normally in broth culture in vitro (data not shown).