TABLE 3.
Protein translational diffusion coefficients (20 C)
|
Dt(10−7cm2/s)
|
||||||
|---|---|---|---|---|---|---|
| Protein* | s† | Mass (kDa) | Calculated | Experimental | References | D‡ |
| BPTI (5PTI) | 1 | 6.5 | 13.66 | 14.4, 14.6 | 56,57 | −6 |
| Cytochrome C (1HRC) | 1 | 12.4 | 11.63 | 11.1–12.1 | 58–61 | 0 |
| Ribonuclease A (7RSA) | 1 | 13.7 | 10.84 | 10.68 | 43 | 2 |
| Lysozyme (2CDS) | 1 | 14.3 | 10.99 | 10.6, 11.2 | 36,37 | 1 |
| a-Lactalbumin (1HFX) | 1 | 14.4 | 10.84 | 10.57, 10.6 | 62,63 | 2 |
| Profilin (1PNE) | 1 | 14.8 | 10.74 | 10.6 | 64 | 1 |
| Myoglobin (1MBO) | 1 | 17.2 | 10.24 | 10.4, 10.5 | 65, 41 | −2 |
| Leghemoglobin (1LH1) | 1 | 17.3 | 10.26 | 10.0 | 66 | 3 |
| b-Lactoglobulin (3BLG) | 1 | 18.4 | 10.07 | 9.7 | 67 | 4 |
| Soybean trypsin inhibitor (1AVU) | 1 | 21.5 | 9.88 | 9.8 | 68 | 1 |
| Cellulase (2ENG) | 1 | 22.0 | 9.63 | 9.8 | 69 | −2 |
| Somatotropin (1HGU) | 1 | 22.1 | 8.84 | 8.88 | 70 | 0 |
| b-Trypsin (1TPO) | 1 | 23.3 | 9.50 | 9.3 | 71 | 2 |
| Trypsinogen (1TGN) | 1 | 24.0 | 9.49 | 9.68 | 48 | −2 |
| Chymotrypsinogen A (2CGA) | 1 | 25.7 | 9.04 | 9.2 | 39 | −2 |
| Elastase (1QNJ) | 1 | 25.9 | 9.22 | 9.5 | 72 | −3 |
| Savinase (1SVN) | 1 | 26.7 | 9.35 | |||
| Subtilysin (1SUP) | 1 | 27.3 | 9.10 | 9.04 | 73 | 1 |
| Carbonic anhydrase B (2CAB) | 1 | 28.7 | 8.84 | 8.89 | 74 | −1 |
| Pepsin (4PEP) | 1 | 34.5 | 8.10 | 8.01, 8.71 | 75,76 | −3 |
| G-actin (1NWK) | 1 | 42.0 | 7.55 | 7.15, 7.88 | 77,78 | 0 |
| Taka-amylase A (6TAA) | 1 | 54.0 | 7.22 | 7.37 | 79 | −2 |
| Human serum albumin (1AO6) | 1 | 69.0 | 6.07 | 5.9–6.32 | 80–82 | −1 |
| Superoxide dismutase (2SOD) | 2 | 32.5 | 8.10 | 8.27 | 83 | −2 |
| b-Lactoglobulin (1BEB) | 2 | 36.7 | 7.74 | 7.27, 7.34, 7.55 | 62, 84, 43 | 5 |
| Concanavalin A (1GKB) | 2 | 51.0 | 6.72 | 6.2 | 85 | 8 |
| Deoxyhemoglobin (2HHB) | 4 | 64.5 | 6.72 | 6.68 | 86 | 1 |
| Oxyhemoglobin A (1HHO) | 4 | 64.5 | 7.02 | 6.78 | 86 | 4 |
| KDPG aldolase (1EUN) | 3 | 69.2 | 6.22 | 5.6 | 66 | 11 |
| Alkaline phosphatase (1ALK) | 2 | 94.7 | 5.92 | 5.7 | 67 | 4 |
| Citrate synthase (1CTS) | 2 | 97.9 | 5.82 | 5.8 | 69 | 0 |
| Concanavalin A (2CTV) | 4 | 102.0 | 5.75 | 5.2, 5.6, 5.8 | 85, 90, 60 | 4 |
| Glucose oxidase (1GPE) | 2 | 133.7 | 5.45 | 5.02, 5.13 | 91, 92 | 7 |
| Canavalin (2CAV) | 3 | 141.0 | 5.32 | 5.10 | 90 | 4 |
| Lactate dehydrogenase (6LDH) | 4 | 145.2 | 5.08 | 4.99 | 30 | 2 |
| Aldolase (1ADO) | 4 | 156.0 | 4.66 | 4.29–4.8 | 93–96 | 3 |
| Glycogen phosphorylase B (1GPB) | 2 | 188.8 | 4.44 | 4.14 | 97 | 7 |
| Nitrogenase MoFe (2MIN) | 4 | 220.0 | 4.41 | 4.0 | 98 | 10 |
| Catalase (4BLC) | 4 | 230.3 | 4.49 | 4.1 | 99,100 | 10 |
| Xanthine oxidase (1FIQ) | 6 | 270.0 | 3.94 | 3.9 | 101 | 0 |
| Glycogen phosphorylase A (1GPA) | 4 | 377.6 | 3.59 | 3.3 | 97 | 9 |
*
Source for the atomic coordinates is the Protein Data Bank file in parentheses.
†
Number of subunits in the protein.
‡
Percent difference between the calculated value and the average of the experimental values.