TABLE 4.
Protein rotational diffusion tensor (20 C)
|
Dr(107s−1)
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Protein | s* | Dr1 | Dr2 | Dr3 | Average | Experimental | References | D† |
| BPTI (5PTI) | 1 | 4.948 | 3.495 | 3.436 | 3.96 | 4.2‡ | 103 | −5.7 |
| Cytochrome C (1HRC) | 1 | 2.794 | 2.512 | 2.293 | 2.53 | 2.4‡ | 104 | 5.4 |
| Ribonuclease A (7RSA) | 1 | 2.401 | 1.810 | 1.716 | 1.98 | 2.01§ | 44 | −1.5 |
| Lysozyme (2CDS) | 1 | 2.638 | 1.860 | 1.802 | 2.10 | 1.7¶, 1.7¶, 2.0‖, 2.2‡ | 36,105,38,106 | 10 |
| a-Lactalbumin (1HFX) | 1 | 2.533 | 1.792 | 1.739 | 2.02 | 1.88‡ | 107 | 7.4 |
| Profilin (1PNE) | 1 | 2.150 | 1.920 | 1.762 | 1.94 | 1.57¶, 2.5‡ | 64,108 | −4.7 |
| Myoglobin (1MBO) | 1 | 1.859 | 1.646 | 1.422 | 1.64 | 1.67** | 42 | −1.8 |
| Leghemoglobin (1LH1) | 1 | 1.990 | 1.606 | 1.445 | 1.68 | |||
| b-Lactoglobulin (3BLG) | 1 | 1.742 | 1.577 | 1.534 | 1.62 | 1.61‖ | 109 | 0.6 |
| Soybean trypsin inhibitor (1AVU) | 1 | 1.692 | 1.459 | 1.414 | 1.52 | 1.32**, 1.33‖ | 110,111 | 14.7 |
| Cellulase (2ENG) | 1 | 1.501 | 1.468 | 1.269 | 1.41 | |||
| Somatotropin (1HGU) | 1 | 1.316 | 0.955 | 0.931 | 1.07 | |||
| b-Trypsin (1TPO) | 1 | 1.513 | 1.331 | 1.217 | 1.34 | 1.16‖ | 112 | 15 |
| Trypsinogen (1TGN) | 1 | 1.492 | 1.350 | 1.214 | 1.35 | |||
| Chymotrypsinogen A (2CGA) | 1 | 1.257 | 1.179 | 1.114 | 1.18 | 1.2‡ | 40 | −1.7 |
| Elastase (1QNJ) | 1 | 1.335 | 1.225 | 1.180 | 1.25 | |||
| Savinase (1SVN) | 1 | 1.359 | 1.307 | 1.226 | 1.30 | 1.26‡, 1.34‡ | 113 | 0 |
| Subtilysin (1SUP) | 1 | 1.248 | 1.208 | 1.127 | 1.19 | |||
| Carbonic anhydrase B (2CAB) | 1 | 1.206 | 1.043 | 1.029 | 1.09 | 1.08‖ | 114 | 0.9 |
| Pepsin (4PEP) | 1 | 1.027 | 0.754 | 0.713 | 0.831 | 0.935** | 110 | −11 |
| G-Actin (1NWK) | 1 | 0.769 | 0.653 | 0.555 | 0.659 | 0.68‖ | 115 | −3 |
| Taka-amylase A (6TAA) | 1 | 0.778 | 0.514 | 0.499 | 0.596 | |||
| Human serum albumin (1AO6) | 1 | 0.394 | .340 | 0.289 | 0.341 | 0.35‖, 0.37††, 0.41††, 0.43†† | 116-119 | −13 |
| Superoxide dismutase (2SOD) | 2 | 1.113 | 0.700 | 0.689 | 0.834 | |||
| b-Lactoglobulin (1BEB) | 2 | 1.008 | 0.586 | 0.570 | 0.721 | 0.75‖, 0.77** | 109, 29 | −5 |
| Concanavalin A (1GKB)§§ | 2 | 0.609 | 0.416 | 0.386 | 0.470 | 0.51‖ | 120 | −8 |
| Deoxyhemoglobin (2HHB) | 4 | 0.536 | 0.470 | 0.459 | 0.488 | 0.51‡ | 121 | −4.3 |
| Oxyhemoglobin A (1HHO) | 4 | 0.631 | 0.517 | 0.549 | 0.555 | 0.56‡ | 122,123 | 0.9 |
| KDPG aldolase (1EUN) | 3 | 0.398 | 0.398 | 0.312 | 0.368 | |||
| Alkaline phosphatase (1ALK) | 2 | 0.450 | 0.278 | 0.271 | 0.333 | 0.31‡ | 124 | 7.4 |
| Citrate synthase (1CTS) | 2 | 0.382 | 02.83 | 0.271 | 0.312 | |||
| Concanavalin A (2CTV) | 4 | 0.304 | 0.291 | 0.290 | 0.295 | 0.28‖ | 120 | 5.3 |
| Glucose oxidase (1GPE) | 2 | 0.297 | 0.244 | 0.231 | 0.258 | |||
| Canavalin (2CAV) | 3 | 0.243 | 0.242 | 0.188 | 0.224 | |||
| Lactate dehydrogenase (6LDH) | 4 | 0.217 | 0.213 | 0.188 | 0.206 | 0.20‖ | 125 | 3 |
| Aldolase (1ADO) | 4 | 0.166 | 0.157 | 0.137 | 0.153 | |||
| Glycogen phosphorylase B (1GPB) | 2 | 0.178 | 0118 | 0.114 | 0.136 | 0.113‡, 0.130‖ | 126,127 | 12 |
| Nitrogenase MoFe (2MIN) | 4 | 0.167 | 0.124 | 0.116 | 0.135 | |||
| Catalase (4BLC) | 4 | 0.149 | 0.141 | 0.122 | 0.137 | |||
| Xanthine oxidase (1FIQ) | 6 | 0.133 | 0.0766 | 0.0727 | 0.0942 | |||
| Glycogen phosphorylase A (1GPA) | 4 | 0.0795 | 0.0741 | 0.0627 | 0.0721 | |||
*
Number of subunits.
†
Percent difference between the calculated value and the average of the experimental values.
‡
Nuclear magnetic resonance.
§
Electric birefringence.
¶
Light scattering.
‖
Fluorescence depolarization.
**
Dielectric relaxation.
††
Anisotropy decay.
§§
Oblate shape.