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. 2006 Jun 9;91(5):1737–1748. doi: 10.1529/biophysj.105.078899

FIGURE 5.

FIGURE 5

[CN]− reversed block by [Au(CN)2]−. (A) Oocytes expressing T338C/Cys-less CFTR were activated and exposed first to 1 mM 2-ME (open circles) and then to 500 μM KCN (shaded triangles). Before [Au(CN)2]−, KCN induced a small and readily reversible inhibition of gCl. After 100 μM [Au(CN)2]− (solid circles) and subsequent washout, exposure to 500 μM KCN induced a rapid increase in gCl, followed by a further increase when KCN was washed from the perfusate. (B) In constant presence of [Au(CN)2]− increasing concentrations of [CN]− shifted the reaction to the unblocked state. After activation, oocytes expressing T338C/Cys-less CFTR were exposed to 1 mM 2-ME (open circles) and then to 1 μM [Au(CN)2]− (solid circles). In constant presence of the pseudohalide, the oocyte was exposed to 20 μM (shaded triangles) and 50 μM (open triangles) KCN. Each exposure of [CN]− rapidly induced a stable increment in conductance, consistent with the increase of the back reaction.