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. 2006 Jun 9;91(5):1737–1748. doi: 10.1529/biophysj.105.078899

FIGURE 8.

FIGURE 8

[Au(CN)2]− irreversibly blocked one of two channels in a patch. (A) Single-channel currents recorded as in Fig. 6 illustrating two current amplitudes, 0.7 pA and 0.2 pA. (B) Exposure to 100 μM [Au(CN)2]− abolished the 0.7-pA current but was without effect on the 0.2-pA current. (C) Removing PKA and ATP from the bath abolished 0.2-pA channel activity. (D) Addition of PKA and ATP to bath restored 0.2-pA channel activity but not 0.7-pA channel activity. (E) 0.7-pA channel activity was restored by exposing the patch to KCN (1 mM) in presence of PKA and ATP. The bottom panels (A1–E1) contain amplitude distributions of current levels for the corresponding traces shown in panels A–E.