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. 2006 Jun 9;91(5):1887–1904. doi: 10.1529/biophysj.106.086561

FIGURE 5.

FIGURE 5

Simulated titrations at low concentrations of ligand B in the calorimetric cell. The concentration of ligand A in the syringe is 300 μM, and the concentrations of macromolecule and ligand B in the calorimetric cell are 20 μM and 10 μM, respectively. The binding enthalpies are ΔHA = 10 kcal/mol and ΔHB = 5 kcal/mol. The cooperativity parameters are α = 0.01 (negative cooperativity (left)), 100 (positive cooperativity (right)), and Δh = 3 kcal/mol. The different titrations have been computed using increasing values of the association constants, but keeping constant the ratio KA/KB: KA = 106 M−1, KB = 104 M−1 (solid squares); KA = 107 M−1, KB = 105 M−1 (open squares); KA = 108 M−1, KB = 106 M−1 (solid circles); KA = 109 M−1, KB = 107 M−1 (open circles); and KA = 1010 M−1, KB = 108 M−1 (solid triangles).