TABLE 1.

Thermodynamic parameters for binding to FNR

	KA (M−1)	KD (M)	ΔG (kcal/mol)	ΔH (kcal/mol)	ΔS (cal/K·mol)
NADP+ → FNRWT	2.6 ± 0.2 × 105	3.8 ± 0.3 × 10−6	−7.4 ± 0.1	−0.4 ± 0.2	23.3 ± 0.8
NADP+ → FNRY303S	1.9 ± 0.2 × 108	5.2 ± 0.5 × 10−9	−11.3 ± 0.1	−8.2 ± 0.2	10.5 ± 0.8
Fd → FNRWT	6.8 ± 0.4 × 105	1.5 ± 0.1 × 10−6	−8.0 ± 0.1	7.8 ± 0.2	52.7 ± 0.8
Fld → FNRWT	2.9 ± 0.3 × 105	3.5 ± 0.3 × 10−6	−7.4 ± 0.1	5.1 ± 0.2	42.0 ± 0.8
Fld → FNRY303S	1.7 ± 0.2 × 105	6.0 ± 0.6 × 10−6	−7.1 ± 0.1	5.4 ± 0.2	42.0 ± 0.8