Table 2.
Cell line | Uninduced | 0.5 μg ml -1 tet | Ratio +tet/-tet |
CL-RFP C2 | 0.3 | 71.8 | 239 |
CL-RFP A2 | 3.3 | 31.7 | 9.6 |
CL-RFP D6 | 0.7 | 25.8 | 36.8 |
CL-RFP D2 | 0.3 | 12.8 | 42.7 |
CL- RFP C5 | 1.1 | 17.5 | 15.9 |
CL-RFP B1 | 0.6 | 2.0 | 3.3 |
Each clone was induced with 0.5 μg ml-1 tetracycline. After 5 days, cells were fixed and analysed on a FacsCalibur. Controls were identical cultures maintained in the absence of tetracycline. The data are presented as percentage of cells registering greater than 6 arbitrary fluorescence units (AFU).