Figure 7.

A. Three images from an image stack (nos. 2, 10 and 17 in a sequence of 20) showing a cell whose volume is entirely occupied within the volume scanned, since the cell body is not visible in ether images 2 or 17. B. seeding of the cell observed in A. using a mouse pointer a seed point is placed within the thresholded selected cell. A seeding region-growing segmentation algorithm is then applied. All contiguous fluorescence above the threshold is accumulated to derive the cell antigen volume.