Structural characterization of the Ftz-F1-containing YAC, ySLH1. (A) Schematic depiction of ySLH1. The gene encoding SF-1, Ftz-F1, is centrally located and flanked by genes for proteosome protein PSMB7 and the orphan nuclear receptor GCNF. Genes are represented by rectangles and the NotI restriction endonuclease used in cloning ySHL1 noted. (B) Schematic representation of the YAC transgenes mapped within transgenic lines 7, 14, and 15. Positions of genes (white bars), Pme I restriction sites, and hybridization probes used in mapping analysis (p01, 5′SF1, S31#3, 3′SF1, SPH10, P400) are indicated on top. Gray bars denote the sequences integrated into each line, with the top one marking the ~153-kb fragment established as sufficient for SF-1 expression.