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. 1999 Feb 16;96(4):1224–1228. doi: 10.1073/pnas.96.4.1224

Figure 1.

Figure 1

Precipitation of coatomer with synthetic peptides corresponding to the COOH termini of p23wt, p23AS and Wbp1p. (A) Peptides used in this study. p23wt represents the cytoplasmic domain of p23, which binds coatomer depending on its dilysine and diphenylalanine motifs, whereas p23AS lacks these residues and therefore does not bind coatomer. Wbp1p represents the cytoplasmic domain of a subunit of the yeast N-oligosaccharyl transferase complex bearing a characteristic KKXX ER-retrieval motif known to bind coatomer. (B and C) Precipitation of coatomer (0.09 μM) with monomeric (B) and dimeric (C) peptides. Only p23wt (■) precipitates the complex, whereas mutated p23 peptide (p23AS, ●) and Wbp1p (▴) have no effect. The error bars indicate standard errors (n = 3).