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Limited proteolysis of coatomer. Proteolysis of coatomer (0.09 μM) with thermolysin (0.008 μM) in the presence of dimerized peptides (50 μM) p23wt-d (A), p23AS-d (B), and Wbp1p-d (C). Western blot analysis of proteolytic fragments of the γ-COP subunit reveals a better susceptibility of this subunit to the protease in the presence of p23wt-d (A, lanes 2 and 3) as compared with p23AS-d (B, lanes 2 and 3 h) and Wbp1p-d (C, lanes 2 and 3 h).
