Figure 5.

Size exclusion chromatography of p23-tail peptides. (A) Monomeric p23wt (p23wt-m: 1 mg/ml; sample corresponds to 20 μl). (B) Dimeric p23wt (p23wt-d: 1 mg/ml; sample corresponds to 20 μl). Peak 1 represents the dimerized form of p23wt-d, and peak 2 represents the monomeric form of p23wt-d. (C) Rechromatography of peak 1 of B (40 μl). The appearance of both peaks 1 and 2 demonstrates an equilibrium between two states of p23wt-d. (D) Dimeric p23wt (p23wt-d: 0.1 mg/ml; sample corresponds to 40 μl). The concentration dependence of the equilibrium of peak 1 and 2 indicates a bimolecular event.