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Effects of E235A and T254A mutations on TRAF6 or TRAF2 association with GST-cyt. GST-cyt or its mutants (E235A or T254A) and Flag-tagged TRAF6 or TRAF2 were transiently expressed in 293T cells and binding assays were performed. Association of Flag-tagged TRAF6 and Flag-tagged TRAF2 with GST-tagged CD40 mutants (Top) and expression level of Flag-tagged TRAF6 and Flag-tagged TRAF2 (Middle) and that of GST-tagged CD40 mutants (Bottom) are shown.
