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. 1999 Feb 16;96(4):1246–1251. doi: 10.1073/pnas.96.4.1246

Figure 5.

Figure 5

Stimulation of PAI-1 expression in BBCE cells by the 16-kDa fragments. Mouse antibovine PAI-1 Western blotting were performed on extracts of BBCE cells prestimulated by 20 nM of the recombinant proteins or were left untreated for control. (A) (Upper) Fragments (16 kDa) stimulate PAI-1 expression; full length hormones do not. (Lower) Quantification. (B) Competition between hGH (22K hGH) and 16K hGH. BBCE cells were treated with 20 nM 16K hGH (16K hGH lanes), with 20 nM hGH (22K hGH lanes), or with 20 nM both 16K hGH and hGH (16K hGH plus 22K hGH lanes) or were untreated (control lanes).