. 2003 May;69(5):2975–2984. doi: 10.1128/AEM.69.5.2975-2984.2003

TABLE 2.

Maximum bacteriocin activities and maximum bacteriocin specific activities for E. faecalis LMG 2333 cultures at different pH values and temperatures as determined with L. lactis IL 1403 as the indicator

Growth conditions		Maximum bacteriocin activity (BU ml⁻¹)	Maximum bacteriocin sp act (BU ml⁻¹ OD₆₀₀⁻¹)^c
pH^a	Temp^b (°C)	Maximum bacteriocin activity (BU ml⁻¹)	Maximum bacteriocin sp act (BU ml⁻¹ OD₆₀₀⁻¹)^c
No pH control		40	27
5.5		40	10
6		640	94
6.5		3,840	420
7		2,560	257
7.5		2,560	251
8		2,560	226
8.5		2,560	253
	20	2,560	307
	25	5,120	548
	30	5,120	602
	35	5,120	607
	37	3,840	420
	40	2,560	351
	45	320	48

All fermentations at different pH values were carried out by using MRS medium with 4% (wt/vol) glucose at 37°C.

All fermentations at different temperatures were carried out by using MRS medium with 4% (wt/vol) glucose at pH 6.5.

OD₆₀₀ was measured spectrophotometrically when the bacteriocin activity was maximum.