TABLE 3.
Stepwise purification of enterolysin A from a culture supernatant of E. faecalis LMG 2333
| Fraction | Vol (ml) | Total A280 | Total activity (103 BU) | Sp act (103 BU A280−1) | Increase in sp act (fold) | Yield (%) |
|---|---|---|---|---|---|---|
| Supernatant | 2,000 | 48,400 | 2,040 | 0.042 | 1 | 100 |
| Precipitation with (NH4)2SO4 | 130 | 3,588 | 2,945 | 0.820 | 20 | 144 |
| Concentration (Centriprep-3) | 37.5 | 3,050 | 2,850 | 0.934 | 22 | 140 |
| Binding to cationic exchanger | ||||||
| First run | 10 | 35.4 | 382 | 10.8 | 257 | 19 |
| Second run | 5 | 6.2 | 153 | 24.8 | 590 | 7.5 |
| Gel filtration | 1 | 1.4 | 50.5 | 29.7 | 707 | 2.5 |