TABLE 2.
Formulations of conventional media used to culture and select H. pylori from mixed microbial samples
| Medium | Proteose peptone (g/liter) | Dex- trose (g/liter) | Sodium chloride (g/liter) | Agar (g/liter) | Di- sodium phos- phate (g/liter) | Calf serum with Fe (ml/liter) | Pep- tamin (g/liter) | Tryp- tone (g/liter) | Yeast extract (g/liter) | Sodium bisulfite (g/liter) | Pantone (g/liter) | Bitone (g/liter) | Beef heart digest (g/liter) | Special peptone (g/liter) | Porcine mucin (g/liter) | Beef extract (g/liter) | Ferrous sulfate (g/liter) | Sodium pyruvate (g/liter) | Urea (g/liter) | Liver digest (ml/liter) | H. pylori selective supple- menta | Campylobacter selective supple- ment Sb |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BHI | 10 | 2 | 5 | 15 | 2.5 | 70 | Yes | |||||||||||||||
| Brucella agar | 1 | 5 | 15 | 10 | 10 | 2 | 0.1 | Yes | ||||||||||||||
| Colombia blood agar base | 1 | 5 | 15 | 10 | 10 | 3 | Yes | |||||||||||||||
| HPSPA mediumc | 5 | 15 | 70 | 5 | 15 | 2 | 5 | 0.5 | 0.5 | 0.6 | Yes | |||||||||||
| Campylobacter agar kit Skirrow | 15 | 5 | 15 | 5 | 2.5 | Yes |
a
H. pylori selective supplement contains vancomycin (10 mg/liter), cefsulodin (5 mg/liter), trimethoprim (5 mg/liter), and amphotericin B (5 mg/liter).
b
Campylobacter selective supplement S contains vancomycin (10 mg/liter), trimethoprim (5 mg/liter), and polymyxin B (2,500 Iu/liter).
c
Formulation described by Jiang and Doyle (9).