TABLE 4.
Growth and inhibition of water-related bacterial strains on conventional media with and without H. pylori selective supplement and on newly formulated HP agar
| Bacterium | Growth on solid media
|
||
|---|---|---|---|
| Conventional media without selective supplementa | Conventional media with selective supplement | HP mediumb | |
| Supplementary | |||
| Acinetobacter | +c | + | − |
| Aeromonas | + | − | − |
| Bacillus | + | − | − |
| Escherichia coli | + | + | − |
| Enterobacter cloacae | + | − | − |
| Enterococcus faecalis | + | − | − |
| Helicobacter | + | + | + |
| Pseudomonas aeruginosa | + | − | − |
| Flora native to well water | |||
| Flavobacterium | + | + | − |
| Serratia | + | − | − |
| Citrobacter | + | − | − |
| Pasteurella | + | + | − |
| Ochrobactrum | + | + | − |
| Rahnella | + | − | − |
Oxoid selective supplement.
HP medium contained (per liter of distilled water) 12 g of special peptone, 5 g of yeast extract, 5 g of beef extract, 5 g of NaCl, 70 ml of calf serum with Fe, 3,500 U of polymyxin B, 7.5 mg of amphotericin B, 10 mg of vancomycin, 5 mg of trimethoprim, 5 mg of cefsulodin, 600 mg of urea, 100 mg of phenol red, 0.8 ml of 1 N HCl, and 15 g of agar. The pH was 6.0 at 22°C. The calf serum with Fe, polymyxin B, amphotericin B, vancomycin, trimethoprim, cefsulodin, and urea were added aseptically after autoclaring and tempering to 50°C. The HCl was added dropwise to cause a color change from red to yellow.
+, growth; −, inhibition.