FIG. 1.

Restriction mapping of the O. oeni ftsH gene. (A) Southern blots were hybridized with either the ftsH probe (left and middle panels) or probe 2 (right panel). The O. oeni genomic DNA was digested with the following enzymes: lane 1, EcoRI; lane 2, BamHI; lane 3, HindIII; lane 4, EcoRI and BamHI; lane 5, EcoRI and HindIII; lanes 6, SalI; lanes 7, EcoRV; lanes 8, EcoRI and SalI. Lanes M, molecular size markers (in base pairs). (B) Physical map of the O. oeni ftsH gene. Restriction enzyme sites: EI, EcoRI; EV, EcoRV; H, HindIII; S, SalI. The location of the ftsH probe is indicated by a grey box above the ftsH gene; the location of probe 2 is indicated by a hatched box below the ftsH gene. Distances between restriction sites are indicated.