FIG. 5.

The O. oeni and B. japonicum ftsH genes protect E. coli from wine toxicity. The E. coli ΔftsH strain AR3291 was transformed with control plasmids (pCR-XL-TOPO [A] or pBAD18-Cm [B]) (light-gray bars) and O. oeni or B. japonicum ftsH gene-containing plasmids (pJPB65 [A] and pRJ5188 [B], respectively) (dark-gray bars). After 3 h of incubation at 25°C with the indicated concentrations of wine, the bacterial cultures were serially diluted and plated. Colonies were counted, and the survival ratios (ratios of CFU observed at a given concentration of wine to those observed without added toxic compounds) were calculated.