TABLE 3.
Recovery of pmoA from soil spiked with pure culturesa
| Recovered pure culture (assay) | Mean no. of target molecules in individual extractionb
|
No. of:
|
|||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | Target molecules | Cellsc
|
||
| Calculated | Added | ||||||
| Methylosinus trichosporium (TYPEII) | 0.9 ± 0.1 | 0.7 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.6 ± 0.1 | 0.3 ± 0.1d | 1.5 ± 0.3 (n = 11) |
| Methylococcus capsulatus (MCOC) | 1.5 ± 0.5 | 4.6 ± 0.8 | 2.2 ± 0.6 | 3.1 ± 0.6 | 2.9 ± 0.7 | 1.4 ± 0.3e | 1.6 ± 0.2 (n = 13) |
| Methylomicrobium album (MBAC) | 0.7 ± 0.2 | 0.7 ± 0.1 | 0.2 ± 0.1 | 0.9 ± 0.1 | 0.6 ± 0.2 | 0.3 ± 0.1e | 0.4 ± 0.1 (n = 13) |
a
Soil was spiked with cells of Methylosinus trichosporium, Methylococcus capsulatus, or Methylomicrobium album.
b
All values are means ± SEs and represent target molecules or cells (107) per milliliter of spiked soil slurry.
c
Numbers of cells were calculated from mean molecule target numbers; 2 pmoA copies per cell were assumed.
d
Calculated cell numbers deviated significantly from added cell numbers (P < 0.01).
e
Calculated numbers of cells were not significantly different from added numbers of cells (P > 0.005).